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. 2018 Oct 12;20(11):1161–1174. doi: 10.1016/j.neo.2018.09.004

Figure 4.

Figure 4

IGF-1 secretion from HFD ASCs induces mammary cancer cell invasion. (A) Met-1 tumor cells were cultured in conditioned media from CD or HFD ASCs then plated on Matrigel-coated Transwells. Invasion was quantified in triplicate (n = 3 tumors/group). (B) MCF-7 cells were cultured in conditioned media from ASCs isolated from obese (BMI >30) or lean (BMI <25) patient reduction mammoplasty tissue (n = ASCs isolated from 3 tissue samples/group). Invasion was quantified in triplicate and represented as a fold change compared with invasion induced by lean BMI ASCs. (C) Relative IGF-1 expression detected using qRT-PCR from CD or HFD ASCs from FVB mice (n = 5 mice/group). (D) Met-1 cells were treated with recombinant mouse (rm) IGF-1 or vehicle and plated on Matrigel-coated Transwells. Invasion was quantified in triplicate (n = 3 experiments). (E) Met-1 cells were treated with conditioned media from HFD ASCs supplemented with control IgG or blocking antibody for IGF-1 and then plated on Matrigel-coated Transwells. Invasion was quantified in triplicate (n = 3 experiments). Bars represent mean ± SD. Magnification bars = 50 μm.