Figure 2.

Experimental Design and LV Used to Determine Safety of the DD System

(A) To determine the safety of DD induction in vivo, animals were injected in the striatum with LV expressing MRFP or YFP-DD and LV expressing GDNF-F-DD and MRFP from two separate expression assets (GDNF-F-DD/MRFP). Half of the GDNF-F-DD/MRFP animals were given regular draining water (OFF), and the other half were given TMP (ON). The last group of animals was injected with processed media from cells transduced only with MRFP transfer plasmid (Sham LV). Twelve or 24 weeks after LV delivery, sera were taken for DD/MRFP ELISA, animals were perfused, and their brains were processed for histology. Expression of the system was assayed using immunohistochemistry for DD, GDNF, and MRFP. Toxicity was assessed using immunohistochemistry for NeuN, CD11B, and CD8. (B) Detailed maps of the transfer vectors used for the experiment.