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. 2018 Oct 8;9:1227. doi: 10.3389/fphys.2018.01227

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Copyright © 2018 Brandenburg, Pawlowitz, Fakuade, Kownatzki-Danger, Kohl, Mitronova, Scardigli, Neef, Schmidt, Wiedmann, Pavone, Sacconi, Kutschka, Sossalla, Moser, Voigt and Lehnart.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Atrial expression of Ca²⁺ handling proteins in different rodent species, pig, and human. (A) Immunoblots comparing the expression of ryanodine receptor 2 (RyR2), sodium-calcium exchanger 1 (NCX), SR Ca²⁺-ATPase 2 (SERCA2), sodium-potassium ATPase α1 subunit (Na,K), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), phospholamban (PLN), and phospholemman (PLM). Insets document the intensity-corrected rabbit NCX and SERCA2 as used for quantification (arrowheads) and additional rabbit-specific antibody cross-reactions (asterisks). Also, the PLN pentameric (p) and monomeric (m) bands are indicated. See Supplementary Figure S4 for full blots and Supplementary Table S1 for clinical patient information. Immunoblot/GAPDH signals (B) and relative protein ratios (C) each normalized to human. M, mouse; Rt, rat; Rb, rabbit; P, pig; H, human. Student’s t-test ^∗P < 0.05, ^∗∗P < 0.01.