Fig. 1.
Effect of NO production of Pg-C-EE in RAW264.7 cells, and HPLC analysis of Pg-C-EE. (A) The level of NO production was determined from culture supernatants of LPS-activated RAW264.7 cells pretreated with Pg-C-EE (i) or l-NAME (ii). (B) The level of NO production was determined from peritoneal macrophage treated with LPS in the presence or absence of Pg-C-EE or l-NAME. (C) Viability of RAW264.7 cells and HEK293 cells incubated with different concentrations of Pg-C-EE determined using the MTT assay. (D) Phytochemical characteristics of ginsenosides in Pg-C-EE were examined by high performance liquid chromatography. *p < 0.05 and **p < 0.01 compared to the normal or control group. l-NAME, Nω-nitiro-l-arginine methyl ester; LPS, lipopolysaccharide; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; Pg-C-EE, ethanolic extract of Panax ginseng berry calyx.