Subject area	Cell biology
More specific subject area	Vascular endothelial cells, inflammation, glycosphingolipids, lipid rafts, fatty acid elongase, ceramide synthase
Type of data	Images and tables
How data was acquired	MALDI-TOF MS and real-time PCR
Data format	Raw and analyzed data
Experimental factors	Vascular endothelial cells stimulated by TNF-α.
Experimental features	Lipid raft–enriched cell membranes were prepared by sucrose density gradient centrifugation in the presence of Triton X-100; purified membrane-associated glycosphingolipids were analyzed by MALDI-TOF MS using α-CHCA as the matrix.
	cDNA was prepared from total cellular RNA, and the expression level of target genes was analyzed by real-time PCR using fluorescent-labeled hydrolysis probes.
Data source location	Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, Tsukuba
Data accessibility	The data are available with this article.
Related research article	T. Okuda, et al. Structural characterization and dynamics of globotetraosylceramide in vascular endothelial cells under TNF-α stimulation. Glycoconj. J. 27 (2010)287–296.[1]