Inhibition of let-7i enhances progesterone (P4)’s effect on the expression of synaptophysin in the penumbra. (A) Representative confocal images of penumbra region staining for synaptophysin (SYP, red) and DAPI (blue). (Magnification: 60×.) (Scale bars: 30 µm.) (B) Quantification of average relative SYP puncta present in each field (n = 3 per group). n.s, not significant; ***P < 0.001 compared with sham; ###P < 0.001 and ##P < 0.01 compared with P4 + scrambled. (C) Representative immunoblots probed for SYP protein. (D) Quantification graph of Syp signal, expressed as a ratio of Gapdh (n = 4 to 5 per group). n.s., not significant; ***P < 0.001, **P < 0.01, *P < 0.05 compared with sham; ##P < 0.01 and #P < 0.05 compared with P4 + scrambled.