Fig. 3.
In vivo infection study in mice immunized with GMMA and conjugate in bivalent formulation. Twelve C57BL/6 mice per group were s.c. immunized at days 0 and 28 at 1 μg OAg/dose per each antigen with Alhydrogel. Seventeen days after the second injection, six mice of each group were challenged intraperitoneally with 104 cfu of S. Typhimurium D23580 or with S. Enteritidis D24954. Twenty-four hours after challenge, mice were killed and spleens and livers collected for bacterial plate counting. (A and B) Summary graphs of anti-OAg IgG geometric mean units (bars) and individual antibody levels (dots). (C and D) SBA titers of single sera collected at day 42 from each group against S. Typhimurium D23580 or S. Enteritidis CMCC4314 strains. (E and F) Bacterial cfu measured in spleens and livers postchallenge. SEn, S. Enteritidis; STm, S. Typhimurium.