Fig. 2.

Streptococcal β-protein protects NK cells from death after infection. (A) Forward-and side-scatter analysis of primary human NK cells in the uninfected state or infected with GBS WT or GBS ΔBAC. (B) Flow cytometry analysis of primary CD56⁺ cells stained with PI to determine cell death without infection or with infection by GBS WT or GBS ΔBAC. (C) Flow cytometry histograms of permeabilized primary human NK cells stained with anti-NLRP3 antibody from two donors. (D) Confocal microscopy images of NK cells stained with anti-NLRP3 antibody without infection or with infection by GBS WT or GBS ΔBAC. The graph quantifies the percentage of NK cells with NLRP3 foci per field of view for each incubation condition. Error bars represent SD.