Fig. 5.

Effect of the phevamines and prephevamine on the spermidine potentiation of the ROS burst and suppression of the calcium burst. (A) Phevamine A suppresses the spermidine potentiation of the flg22-induced ROS burst in N. benthamiana, but does not affect the flg22-induced ROS burst directly. Leaf disks were treated with 50 nM flg22, with or without spermidine at 400 µM, and with or without phevamine A at 400 µM. (B) Phevamine A and prephevamine suppress the spermidine potentiation in N. benthamiana, but phevamine B does not. Leaf disks were treated with flg22 at 10 nM, spermidine, phevamines, and prephevamine were used at 300 µM. (C) In Arabidopsis, phevamine A suppresses the spermidine potentiation, while prephevamine does not. Leaf disks were treated with flg22 at 10 nM, spermidine and phevamines were used at 300 µM. (D) Phevamine A does not affect the flg22-induced Ca²⁺ burst, or the reduction of this burst by spermidine. Leaf disks were treated with flg22 at 20 nM, spermidine and phevamine A were used at 300 µM. Error bars represent SEs. PHVA, phevamine A; PHVB, phevamine B; pPHV, prephevamine; RLU, relative luminescence units; Spd, spermidine. All experiments presented here were repeated at least three times.