Fig. 6.

Survival of BALB/c mice infected i.v. with C. albicans. (A) Percentage of mouse weight postinfection. Mice (n = 10 per group) received PBS, fluconazole (200 mg⋅kg⁻¹⋅d⁻¹), CE (100 mg⋅kg⁻¹⋅d⁻¹), or CE (500 mg⋅kg⁻¹⋅d⁻¹) via i.p. injection over a 7-d period after i.v. infection with C. albicans (Methods). The weight of the mice was recorded every day for the duration of the experiment (40 d), and mice were killed by CO₂ asphyxiation when they showed ≥20% weight loss or signs consistent with disseminated candidiasis. Each mouse showed weight loss for at least four consecutive days postinfection. Black lines represent each individual within groups, red lines the mean of each group, and a blue line represents an outlier in the PBS group that did not show signs of infection (i.e., weight loss). (B) Organ burden analysis calculated in colony forming units (CFU) per gram of tissue. One-way ANOVA test was performed to determine statistical significance (kidney: F = 17.82, P < 0.0001; spleen: F = 18.26, P < 0.0001; liver: F = 14.87, P < 0.0001; lung: F = 8.93, P = 0.0002; and brain: F = 17.7, P < 0.0001; degrees of freedom = 3 for treatments and 35 for residuals), followed by Tukey test (α = 0.05, **P < 0.01; ***P < 0.001; ****P < 0.0001).