Fig. 7.

Role of VCAM-1–mediated β1-integrin activation in PC differentiation. (A and B) Flow cytometry to identify CD138⁺Blimp-GFP^int PB and CD138⁺Blimp-GFP^hi PC in spleen of Mzb1^+/+Prdm1^+/gfp and Mzb1^−/−Prdm1^+/gfp mice that were administered 100 µg of anti–VCAM-1 antibody or rat IgG intravenously 24 h after TNP-LPS immunization and 2 d before analysis. (B) Mean (±SD) frequencies of PB (Upper) and PC (Lower). (C) ELISpot analysis to detect TNP⁺IgM⁺ -secreting PB and PC cells in spleen. Data are representative of three different experiments; n = 4–6. *P < 0.05, **P < 0.01, ***P < 0.001. (D–F) Flow cytometry and ELISpot analysis to detect CD138⁺Blimp-GFP^int PB and CD138⁺Blimp-GFP^hi PC in the BM of TNP-LPS–immunized and anti–VCAM-1–treated Mzb1^+/+Prdm1^+/gfp and Mzb1^−/−Prdm1^+/gfp mice as in A–C.