Sleep/wake episode durations and EEG power spectra of S551D mutants. (A) Episode durations of wakefulness, NREMS and REMS. n = 10 for each genotype. *P < 0.05, two-tailed unpaired t test and Mann–Whitney U test. (B) EEG power spectra in wakefulness, NREMS, and REMS states. n = 10 for each genotype. **P < 0.01, ***P < 0.001, ****P < 0.0001, two-way repeated-measure ANOVA followed by Bonferroni’s multiple comparisons test. Wake: the main effect of genotype, F(1,
18) = 6.562, *P = 0.0196. NREMS: the main effect of genotype, F(1,
18) = 1.459, P = 0.2427. REMS: the main effect of genotype, F(1,
18) = 4.451, *P = 0.0491. (C) NREMS δ-power density of S551D mutants. n = 10 for each genotype. *P < 0.05, **P < 0.01, ***P < 0.001, two-way repeated-measure ANOVA followed by Bonferroni’s multiple comparisons test. The main effect of genotype, F(1,
18) = 14.285, **P = 0.0014. NREMS/REMS/wakefulness stages are assigned in 20-s epochs. Data are presented as the mean ± SEM.