Fig. 4.
IRIS inhibits HIF-1α degradation via the AKT/GSK-3β pathway. (A) Effects of various kinase inhibitors on HIF-1α protein expression in M231 cells. LY294002 is a PI3K inhibitor; CHIR98014 is a GSK-3β inhibitor. (B) Western blot showing expression levels of various proteins in BC3-p53KD cells before and after IRIS depletion. (C) Effects of IRIS expression on levels of HIF-1α, PTEN, and phospho-AKT in A549 and ACHN cells. (D) Effects of IRIS expression on the expression of the downstream PI3K pathway and certain other targets in HME cells.