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. 2018 Jul 26;155(3):387–395. doi: 10.1111/imm.12981

Figure 2.

Figure 2

Low‐molecular‐mass hyaluronan (LMMHA) (200 000 MW) stimulates the activation of Akt1 kinase. Phosphorylation of Akt1 in lung homogenates was assessed by Western blot analysis 6 hr or 12 hr after intratracheal administration of LMMHA (200 000 MW, 65 mg/kg) (a). Phosphorylation of Akt1 in whole lung neutrophils was assessed 6 hr after LMMHA administration (b). Phosphorylation of p38 and ERK1/2 in whole lung neutrophils 6 hr after LMMHA (c). Mouse whole lung neutrophils were isolated as described in the Materials and methods.