Figure 3.

Increased complement activation and macrophage activation in the spinal cords of CD93‐deficient mice treated for antibody‐dependent autoimmune encephalomyelitis (ADEAE). (a) The ADEAE is more severe and acute than EAE and through the capacity of the Z12 monoclonal antibody drives a robust activation of the complement system. We stained for C3 (see Table 1) and for C1q and found a more robust complement activation/opsonization in CD93^−/− mice when compared with wild‐type (WT). Triple immunostaining for Z12, tomato lectin (TL) and C1q. Magnification at 200 × in top six panels, and × 600 for bottom six panels depicting the inset areas (b). Panels (a, b) representative data obtained from tissue sections from three different brains from either WT or knockout (KO) animals of comparable clinical scores.