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. 2018 Sep 14;37(20):e99395. doi: 10.15252/embj.201899395

Figure 3. Dissecting the mitotic interactome of BCL9.

Figure 3

  1. Volcano plot of the BCL9 mitotic interactome by the SILAC proteomics approach. Red dashed line indicates a strict cut off by a normalised SILAC H/L ratio > 2.
  2. DAVID functional annotation analysis of enriched biological process (BP) by the potential mitotic BCL9 interactome (normalised SILAC H/L ratio > 1.5).
  3. An independent immunoprecipitation assay for validation of potential BCL9 interactors in both asynchronised and mitotic cells. The relative binding of CLTC with BCL9 is quantified and labelled on top of the CLTC blot.
  4. Immunoprecipitation assay of the endogenous BCL9 and Axin1 interaction in synchronised HeLa cells, from G1S to G2M. P‐H3S10, a mitotic marker indicating mitosis.
Data information: See also Fig EV3 and Table EV4.Source data are available online for this figure.