Skip to main content
. 2018 Sep 14;37(20):e99395. doi: 10.15252/embj.201899395

Figure EV4. BCL9 regulates mitotic destruction complex and competes with clathrin for LRP6/Axin1 complex binding (related to Fig 4).

Figure EV4

  1. Immunoprecipitation analysis of endogenous Axin1 or APC interaction with CLTC or β‐catenin during different stages of cell cycle by thymidine synchronisation. Thy R (h) indicates hours post‐double thymidine release.
  2. The relative binding was determined for (A).
  3. A rescue experiment by investigating the effect of re‐expression of BCL9‐Flag in BCL9 knockdown cells on the Axin1 interaction with CLTC or β‐catenin.
  4. The immunoprecipitation analysis of BCL9 and CLTC in HAP1 parental and BCL9 KO cells.
  5. The input for immunoprecipitation analysis in Fig 4E.
  6. An additional data for the validation of ΔHD1 which is important for interaction with destruction complex, but is not well confirmed in (E).
  7. Immunoblotting analysis of the effect of BCL9 and two truncates re‐expression on mitotic Wnt signalling activity by detecting mitotic Wnt target proteins.

Source data are available online for this figure.