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Confirmation of E. coli O157:H7 preconcentrating and DNA purifying efficiency using either PCR with gel electrophoresis and qPCR; (a) Gel electrophoresis of PCR products (eae gene) from 10 mL PBS containing E. coli O157:H7 at different concentrations (1–103 CFU/mL) before preconcentration; (b) after preconcentration; (c) after bacterial preconcentration and DNA purification; (d–f) qPCR results of (a–c), respectively.
