Figure 3.
Characterization of extracellular vesicle concentration enhancement for 5.0 × 107 particles/1 mL (LOD), 5.0 × 108 particles/1 mL, and 5.0 × 109 particles/1 mL at 45 V/cm on a SuperEpoxy 3 microarray slide without antibody. (a) bright fluorescent plug inside the channel indicating EVs near the conductive polymer membrane—size continued to increase as a function of concentration time. (b) Fluorescence signal intensity curves of 5.0 × 107 particles/1 mL (LOD), 5.0 × 108 particles/1 mL, and 5.0 × 109 particles/1 mL. EV concentrations of 5.0 × 107 particles/1 mL (LOD), 5.0 × 108 particles/1 mL, and 5.0 × 109 particles/1 mL increased by ~100-fold within 30 min (ND 1 filter and 1 s exposure time).