Table 1. Cytotoxicity of A1B4 on the Different Life Cycle Stages of T. cruzi and Vero Cells.
| IC50 (μM)a,b (SIc) |
||||
|---|---|---|---|---|
| compound | epimastigotes | trypomastigotes | amastigotes | Vero cells |
| A1B4 | 23 ± 3.8 (>9) | 17.8 ± 2.29 (>11) | 13.1 ± 1.28(>15) | >200 ± 6 |
| BZNd | 18.16 ± 5.13 (2) | 27.07 ± 4.23 (1) | 3.92 ± 1.24 (8) | 30.17 ± 12 |
| NFXe | 8 ± 3 (14) | 7 ± 2 (16) | 3 ± 1.5 (38) | 115 ± 12 |
a
The results are averages of three separate determinations.
b
IC50 is the concentration required to give 50% inhibition, calculated by nonlinear regression analysis from beta-galactosidase activity at the used concentrations (0–200 μM).
c
Selectivity index (SI) is the ratio between IC50 on Vero cell toxicity and IC50 activity of extracellular or intracellular forms of the parasite.
d
MTT assay on Vero cells incubated 72 h with BZN (0–250 μM).
e
Values obtained from the literature.31 BZN, benznidazol; NFX, nifurtimox (reference drugs for the treatment of Chagas disease).