Fig. 2. Glyco-motif targeting. Examples of chemo-enzymatic labelling using a given glycosyltransferase (named in red), its associated nucleotide sugar, and the corresponding acceptor glycol-motif. Step 1: an unnatural monosaccharide is transferred from its activated form onto the targeted glycan through the action of a glycosyltransferase. Step 2: the introduced reporter (x) is reacted with a probe bearing a complementary bioorthogonal function (y) allowing its detection.
