Figure 4.

Percentage of spike events and dwell times histograms. (a) Percentage of spike events, for various conditions. Left bar shows a much higher number of events with spikes than in all controls where one of the components was lacking or where the guide RNA was mutated or nonfitting to the target DNA sequence. (b) Bar graph showing the percentage of spikes larger than 3I₀ for the dCas9-DNA sample and the DNA-only control at three different voltages. At all voltages, the percentage of spike events in the dCas9-DNA sample significantly exceeds the presence of spike events of the DNA-only events (that are due to knots and folds). (c) Histogram of the dwell times of the dCas9-DNA at 100 mV (purple), 200 mV (blue), and 300 mV (red). At 300 and 200 mV, the distribution times are clustered near 0.5 and 1 ms, respectively, whereas a broadening of the dwell times is observed at 100 mV due to interactions with the pore.