Figure 8.

Setting up co-culture, (A–C) the creation of a 3 × 3 array of yeast cells; (D–F) a single S. cerevisiae yeast (dashed arrow) and single E. coli bacteria (solid arrow) tweezed through meandering channel in PDMS device into isolation chamber; (G) two yeast cells deposited by optical tweezers into PDMS isolation chamber at t = 0; (H) t = 1 day showing three yeast cells and bacterial contamination; (I) t = 5 days, two yeast cells are visible, the third is at the edge of the chamber and bacteria are numerous.