Table 2.
Effects of alanine substitutions in RAMP1:CLR on AM2/IMD activation of cAMP signaling
*, p < 0.05.
| Receptor mutant | WT pEC50 ± S.E. (n) | Mutant pEC50 ± S.E. (n)a | -Fold change | Mutant Emax (% of WT) ± S.E. (n)b |
|---|---|---|---|---|
| CLR T37A | 8.43 ± 0.10 (3) | 6.91 ± 0.06* (3) | 33 | 98.9 ± 3.08 (3) |
| CLR W72A | 8.33 ± 0.08 (3) | 6.51 ± 0.23* (3) | 146 | 121.1 ± 17.1 (3) |
| CLR F92A | 8.50 ± 0.08 (3) | 6.22 ± 0.14* (3) | 191 | 55.9 ± 19.5 (3) |
| CLR D94A | 8.47 ± 0.03 (3) | 7.35 ± 0.11* (3) | 12 | 131.9 ± 8.52 (3) |
| CLR F95A | 8.36 ± 0.05 (3) | 6.21 ± 0.16* (3) | 143 | 108.1 ± 15.2 (3) |
| CLR H114A | 8.27 ± 0.09 (3) | 6.89 ± 0.07* (3) | 24 | 98.2 ± 21.2 (3) |
| CLR R119A | 8.53 ± 0.14 (5) | 6.61 ± 0.04* (5) | 83 | 100.9 ± 22.2 (5) |
| CLR W121A | 8.29 ± 0.10 (3) | <5.3 (3) | >1,000 | Undeterminable |
| CLR Y124A | 8.41 ± 0.04 (3) | 6.63 ± 0.09* (3) | 60 | 37.1 ± 7.49* (5) |
| RAMP1 W84A | 8.38 ± 0.07 (4) | 7.32 ± 0.15* (3) | 12 | 81.3 ± 9.52 (3) |
a Statistical significance of pEC50 values was determined by a paired t test comparing the mutant pEC50 with the WT pEC50.
b Statistical significance of Emax values was determined by a ratio paired t test comparing the mutant Emax with the WT Emax before normalization.