Figure 2.
Receptor binding and biological activity of IFNϵ and IFNκ preparations. A, receptor binding of IFNϵ and IFNκ, as well as IFNα2 and IFNω, measured as percent RO. RO corresponds to the SPR RU observed upon IFN injection over an IFNAR Biacore chip surface, divided by the theoretical RUmax of the surface, multiplied by 100. B, dose-response curves for IFNϵ, IFNκ, IFNα2, and IFNω-mediated activation of the IFI6 gene reporter in HL116 cells. The color code for the dose-response curves is the same as the receptor binding analysis in A.