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Neutralization of IFN biological activity, by antibodies, soluble IFNARs, and the viral antagonist B18R. IFNs and antagonists were incubated with reporter cells for 18 h, followed by measurement of ISG54 reporter activity. All measurements are normalized relative to the IFN control, which is the average of 4 measurements corresponding to IFN alone and IFN + three isotype antibody controls.
