Table 2.

Surface plasmon resonance-derived binding constants and ISFG3 assay EC₅₀ values

—, not determined.

		ka	kd	KD
		m−1 s−1	s−1	m
IFNα2	IFNAR1	—	—	2.3 (± 0.5) × 10−6
EC50 = 11 pm (±3 pm)	IFNAR2	5.9 (± 0.4) × 106	0.031 (± 0.002)	5.3 (± 0.2) × 10−9
	IFNAR1/2	8 (± 1) × 106	4.2 (± 0.4) × 10−4	54 (± 3) × 10−12
IFNϵ	IFNAR1	—	—	2.2 (± 0.3) × 10−6
EC50 = 60 nm (±26 nm)	IFNAR2	4 (± 3) × 105	0.01 (± 0.01)	70 (± 16) × 10−9
	IFNAR1/2	3.3 (± 0.8) × 105	9 (± 1) × 10−4	3.5 (± 0.8) × 10−9
IFNω	IFNAR1	—	—	0.5 (± 0.1) × 10−6
EC50 = 6 pm (±1 pm)	IFNAR2	7 (± 2) × 107	0.01 (± 0.07)	237 (± 37) × 10−12
	IFNAR1/2	3 (± 2) × 108	4 (± 2) × 10−3	25 (± 8) × 10−12
IFNκ	IFNAR1	—	—	0.34 (± 0.13) × 10−6
EC50 = 22 nm (±16 nm)	IFNAR2	1.2 (± 0.2) × 105	2.1 (± 0.3) × 10−3	21 (± 4) × 10−9
	IFNAR1/2	4 (± 2) × 105	8 (± 1) × 10−4	2 (± 1) × 10−9
IFNκ-M2	IFNAR1/2	7 (± 2) × 105	3 (± 1) × 10−4	0.4 (± 0.3) × 10−9
EC50 = 4 nm (±2 nm)
IFNα1	IFNAR1	—	—	0.4 × 10−6
EC50 = 258 pm (±73 pm)	IFNAR2	5 (± 0.1) × 105	0.18 (± 0.02)	353 (± 83) × 10−9
	IFNAR1/2	1.8 (± 0.2) × 106	4 (± 2) × 10−4	222 (± 92) × 10−12
IFNα1-M1	IFNAR1	—	—	0.2 × 10−6
EC50 = 26 pm (±10 pm)	IFNAR2	2.4 (± 0.1) × 106	0.06 (± 0.01)	26.0 (± 0.4) × 10−9
	IFNAR1/2	5.0 (± 0.1) × 106	3.5 (± 0.5) × 10−4	70 (± 9) × 10−12
IFNϵ	B18R	1.5 × 105	3.1 × 10−5	2.10 × 10−10
IFNα1	B18R	5.5 × 105	2.3 × 10−5	4.2 × 10−11
IFNα1-M1	B18R	2.2 × 106	2.3 × 10−5	1.1 × 10−11
IFNα4	B18R	5.8 × 106	1.5 × 10−5	2.6 × 10−12
IFNα14	B18R	1.1 × 107	2.1 × 10−5	1.9 × 10−12