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. 2018 Aug 21;293(41):15765–15776. doi: 10.1074/jbc.RA118.002611

Figure 2.

Figure 2.

Competition binding of R3 B1-22R variants at RXFP3. The effect of representative R3 B1-22R substitutions including Ala substitutions (A), Arg variants (B), helix breakers (Pro) or helix promotors (Aib) (C), and side-chain and backbone cyclization (D) on the ability to compete for binding with europium-labeled R3 B1-22R is shown. Data are presented as mean ± S.E. (error bars) from a minimum of three independent experiments.