Table 2.
Ubiquitin-modified lysine residues of TDP-43 and ubiquitin, identified by MS/LC analysis in N- and C-terminally His6-tagged TDP-43 purified by Ni-NTA affinity chromatography
Coverages of TDP-43 in analyzed samples are specified as percentage values in parentheses.
| DMSO treatment, analyzed band HMW | DMSO treatment, analyzed band TDP-43 mono | MG-132 treatment, analyzed band HMW | MG-132 treatment, analyzed band TDP-43 mono | ||
|---|---|---|---|---|---|
| TDP-43 | His6-TDP-43 | Lys-84, Lys-181, Lys-263 (45%) | Lys-84, Lys-181 (46%) | Lys-84, Lys-95, Lys-160, Lys-181, Lys-263 (52%) | Lys-84, Lys-95, Lys-160, Lys-181, Lys-263 (60%) |
| TDP-43-His6 | Lys-102, Lys-181 (44%) | Lys-114, Lys-181 (83%) | Lys-84, Lys-95, Lys-102, Lys-160, Lys-181, Lys-263 (62%) | Lys-84, Lys-95, Lys-102, Lys-114, Lys-160, Lys-181, Lys-263 (72%) | |
| Ubiquitin | His6-TDP-43 | Lys-48, Lys-63 | Lys-48 | Lys-48, Lys-63 | Lys-48, Lys-63 |
| TDP-43-His6 | Lys-48, Lys-63 | Lys-11, Lys-48, Lys-63 | Lys-48, Lys-63 | Lys-48, Lys-63 | |