Figure 9.
Effect of temperature on the growth of cells harboring the phosphorylation-deficient and -mimicking alleles of NEM1 and SPO7. Yeast strains containing the indicated serine-to-alanine (A) or -aspartate (B) mutations in chromosomal NEM1 and SPO7 were grown at 30 °C to saturation in YEPD medium. The saturated cultures were diluted to a density of 0.67 at A600 nm, followed by 10-fold serial dilutions. The diluted cultures were spotted (2.5 μl) onto YEPD plates and incubated for 3 days at 30 and 37 °C. The data are representative of three independent experiments.