Table 2.
Strains used in this work
| Strain | Genotype or relevant characteristics | Source/Reference |
|---|---|---|
| E. coli | ||
| DH5α | F− φ80dlacZΔM15Δ (lacZYAargF)U169 deoR recA1 endA1 hsdR17 (rk− mk+) phoA supE44 λ−thi-1 gyrA96 relA1 | Ref. 70 |
| BL21(DE3)pLysS | F− ompT hsdSB (rB−mB−) gal dcm (DE3) pLysS | Novagen |
| BL21(DE3) | F− ompT hsdSB (rB−mB−) gal dcm | Invitrogen |
| S. cerevisiae | ||
| RS453 | MATa ade21 his311,15 leu23,112 trp11 ura352 | Ref. 52 |
| Derivatives | ||
| SS1010 | nem1Δ::HIS3 spo7Δ::HIS3 | Ref. 1 |
| SS1002 | nem1Δ::HIS3 | Ref. 1 |
| WMY161 | nem1Δ::URA3 | This study |
| WMY162 | NEM1-PtA | This study |
| WMY163 | NEM1(S140A)-PtA | This study |
| WMY164 | NEM1(S140D)-PtA | This study |
| WMY165 | NEM1(S210A)-PtA | This study |
| WMY166 | NEM1(S210D)-PtA | This study |
| WMY167 | NEM1(S140A/S210A)-PtA | This study |
| WMY168 | NEM1(S140D/S210D)-PtA | This study |
| GHY67 | spo7Δ::URA3 | This study |
| GHY68 | NEM1-PtA spo7Δ::URA3 | This study |
| GHY69 | NEM1(S140A)-PtA spo7Δ::URA3 | This study |
| GHY70 | NEM1(S140D)-PtA spo7Δ::URA3 | This study |
| GHY71 | NEM1(S210A)-PtA spo7Δ::URA3 | This study |
| GHY72 | NEM1(S210D)-PtA spo7Δ::URA3 | This study |
| GHY73 | NEM1(S140A/S210A)-PtA spo7Δ::URA3 | This study |
| GHY74 | NEM1(S140D/S210D)-PtA spo7Δ::URA3 | This study |
| GHY75 | SPO7(S28A) | This study |
| GHY76 | SPO7(S28D) | This study |
| GHY77 | NEM1-PtA SPO7(S28A) | This study |
| GHY78 | NEM1-PtA SPO7(S28D) | This study |
| GHY79 | NEM1(S140A)-PtA SPO7(S28A) | This study |
| GHY80 | NEM1(S140D)-PtA SPO7(S28D) | This study |
| GHY81 | NEM1(S210A)-PtA SPO7(S28A) | This study |
| GHY82 | NEM1(S210D)-PtA SPO7(S28D) | This study |
| GHY83 | NEM1(S140A/S210A)-PtA SPO7(S28A) | This study |
| GHY84 | NEM1(S140D/S210D)-PtA SPO7(S28D) | This study |