Table 3.
Plasmids used in this work
| Plasmid | Relevant characteristics | Source/Reference |
|---|---|---|
| YCplac111-GAL1/10-NEM1-PtA | NEM1PtA under control of GAL1/10 promoter in CEN/LEU2 vector | Ref. 4 |
| Derivatives | ||
| pWM201 | NEM1(S140A) | This study |
| pWM202 | NEM1(S210A) | This study |
| pWM203 | NEM1(S140A/S210A) | This study |
| pRS313-GAL1/10-SPO7 | SPO7 under control of GAL1/10 promoter in CEN/HIS3 vector | Ref. 52 |
| pRS314-GAL1/10-SPO7 | SPO7 under control of GAL1/10 promoter in CEN/TRP1 vector | This study |
| Derivatives | ||
| pWM211 | SPO7(S28A) | This study |
| pWM212 | SPO7(S41A) | This study |
| pWM213 | SPO7(S42A) | This study |
| pWM214 | SPO7(S41A/S42A) | This study |
| YCplac111-NEM1-PtA | NEM1PtA inserted into the CEN/LEU2 vector | S. Siniossoglou |
| Derivatives | ||
| pWM204 | NEM1(S140A) | This study |
| pWM205 | NEM1(S140D) | This study |
| pWM206 | NEM1(S210A) | This study |
| pWM207 | NEM1(S210D) | This study |
| pWM208 | NEM1(S140A/S210A) | This study |
| pWM209 | NEM1(S140D/S210D) | This study |
| pRS426 | Multicopy E. coli/yeast shuttle vector with URA3 | Ref. 88 |
| pRS416 | Single-copy E. coli/yeast shuttle vector with URA3 | Ref. 89 |
| pRS415 | Single-copy E. coli/yeast shuttle vector with LEU2 | Ref. 89 |
| pGH443 | SPO7 inserted into pRS415 | This study |
| Derivatives | ||
| pGH444 | SPO7(S28A) | This study |
| pGH445 | SPO7(S28D) | This study |