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. 2018 Aug 30;293(41):15889–15900. doi: 10.1074/jbc.RA118.004991

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© 2018 Rozman Grinberg et al.

Author's Choice—Final version open access under the terms of the Creative Commons CC-BY license.

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Figure 3. — GSH-dependent RNR activity of F. ignava NrdB WT and mutant proteins. CDP was used as substrate, and 3 mm ATP was used as effector. A, RNR activity measured as NADPH consumption in presence of 0.5 μm NrdB. B, GSH-dependent NADPH consumption of the WT (●) and the C15S (○) NrdB. C, GSH-dependent specific activity measured as dCDP formation. Inset, DTT-dependent (10 mm) specific activity measured as dCDP formation. GSH concentrations (4 and 10 mm) are indicated in A and C. Assays in A and C were performed in triplicate with standard deviations shown.