Figure 7.

Size-exclusion chromatography analysis of F. ignava RNR components in the presence of nucleotides. A, 5 and 10 μm of the NrdA subunit was analyzed in the presence of 3 mm ATP or 100 μm dATP or without effector. B, corresponding analysis of the NrdB subunit. In this case the experiment without effector was performed at 1.25, 2.5, 5, and 10 μm protein. The position of the peaks indicate a larger size than expected, which is typical for elongated proteins, and the interpretation above the peaks is based on a comparison with the GEMMA results. C, analysis of the combination of both subunits. Each subunit was used at 10 and 20 μm concentration except in the experiment without effector, where only 10 μm was used.