Figure 1.

PRV UL37 and HSV-1 UL37N constructs and properties. A, schematic of PRV UL37 WT with domain boundaries labeled based on crystal structure determination of PRV UL37N (PDB code 4K70). B, schematics of PRV UL37FL (green/blue, Gene ID: 2952491), PRV UL37N (green), HSV1-UL37N (orange, Gene ID: 2703358), and PRV UL37C (blue). Linker resulting from cleavage of the His₆-SUMO tag with PreScission protease (pink) and linker preceding Strep-II tag (orange) is shown. C, theoretical molecular mass (MM) for all constructs as calculated from amino acid sequence; presence (✓) or absence (×) of crystal formation; MM as calculated from SEC (SEC MM), SEC-SAXS (SEC-SAXS MM), or SEC-MALS (SEC-MALS MM); and melting temperature as determined from the Thermofluor assay (Thermofluor TM).