Figure 3.

Limited proteolysis of PRV UL37C(478–919)-StII. A and B, limited proteolysis of PRV UL37C(478–919)-StII using either chymotrypsin (A) or trypsin (B) with decreasing amounts of protease. Solid arrows, original protein; open arrows, digestion products. C, secondary structure prediction for proteolytically sensitive C-terminal tail as calculated by PsiPred (http://bioinf.cs.ucl.ac.uk/psipred) (49). Blue cylinders, predicted helices. Cleavage sites for chymotrypsin and trypsin are indicated in blue and purple, respectively, based on MALDI-TOF data (Fig. S2). Linker (orange) and Strep-II tag (gray) are included.