Table 1.
SAXS structural parameters
| UL37C(478–919)-StII | UL37C(478–884) | HSV-1 UL37N | |
|---|---|---|---|
| Guinier analysis | |||
| I(0) (cm−1)a | 0.017 ± 0.00007 | 0.0081 ± 0.00007 | 0.02786 ± 0.00008 |
| Rg (Å)b | 41.97 ± 0.95 | 38.86 ± 0.51 | 33.34 ± 0.14 |
| qmin (Å−1)c | 0.00855 | 0.0108 | 0.00912 |
| qmaxRgd | 1.29208 | 1.30719 | 1.29265 |
| Coefficient of correlation, R2e | 0.9909 | 0.9695 | 0.9968 |
| P(r) analysis (forced to 0/not forced)f | |||
| I(0) (cm−1) | 0.017 ± 0.00008/0.019 ± 0.0004 | 0.0078 ± 0.00004/0.0085 ± 0.0002 | 0.02785 ± 0.00005 |
| Rg (Å) | 43.00 ± 0.17/50.2 ± 0.30 | 39.34 ± 0.16/47.63 ± 0.38 | 33.81 ± 0.065 |
| Dmax (Å)g | 140/250 | 125/250 | 120 |
| q range (Å−1)h | 0.007–0.2808/0.007–0.2808 | 0.007–0.2808/0.007–0.2808 | 0.007–0.2808 |
| χ2 (total estimate from GNOM)i,j | 1.169 (0.80)/1.087 (0.68) | 1.112 (0.80)/1.055 (0.66) | 1.106 (0.88) |
| DAMAVER | |||
| Symmetry,k anisotropy assumptionsl | P1, none | P1, none | P1, none |
| Models averagedm (omitted)n | 10 (0) | 10 (0) | 10 (0) |
| Mean NSDo (S.D.) | 0.619 (0.014) | 0.660 (0.017) | 0.644 (0.014) |
| χ2 rangep | 1.186–1.197 | 1.172–1.175 | 1.013–1.015 |
| Resolutionq (from SASRES (48)) (Å) | 41 ± 3 | 42 ± 3 | 38 ± 3 |
a I(0) = scattering intensity at zero angle.
b Radius of gyration.
c qmin = lowest q value used in the Guinier analysis.
d Maximum q value used in the Guinier analysis times Rg.
e Linear fit of the Guinier plot.
f P(r) forced to 0 at Dmax in DATGNOM/P(r) not forced to 0.
g Maximum dimension calculated from P(r).
h q values used in P(r) plot.
i χ2 fit of P(r) to data.
j Total quality estimate from GNOM.
k Symmetry used for DAMMIN input.
l Prolate, oblate, or none for DAMMIN input.
m Number of DAMMIN models averaged using DAMAVER.
n Number of DAMMIN models omitted from DAMAVER if NSD > mean + 2 × S.D.
o Mean NSD for each model used in DAMAVER average.
p Range of χ2 values for input DAMMIN models.
q Resolution of final filtered average from DAMAVER.