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. 2018 Aug 21;293(41):15748–15764. doi: 10.1074/jbc.RA118.003116

Figure 2.

Figure 2.

Analysis of purified huPrP by CD spectroscopy (A) and AFM (B) and of Aβ(1–42)oligo by AFM (C). huPrP(23–230) (A, black) and huPrP(90–230) (A, blue) show predominantly α-helical CD spectra, whereas the N-terminal huPrP(23–144) (A, red) is present in random-coil conformation (MRE, mean residue ellipticity; deg, degrees). Shown are 1-μm2 AFM images of 200 nm huPrP(23–144) (B) or 800 nm Aβ(1–42)oligo (C). Scale bars, 200 nm. huPrP(23–144) forms a thin film on the mica surface, not higher than 1–2 nm (B). The generated Aβ(1–42)oligo species are seen as spherical particles with heights ranging from 1 to 6 nm (C).