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. 2018 Aug 21;293(41):15748–15764. doi: 10.1074/jbc.RA118.003116

Figure 9.

Figure 9.

Interference of the Aβ(1–42)oligo–huPrP(23–144) interaction by RD2D3-FITC. Shown is the distribution of 80 μm Aβ(1–42), 40 μm huPrP(23–144), and 40 μm RD2D3-FITC after sucrose DGC for different orders of RD2D3-FITC and huPrP(23–144) addition. A, C, and E, Aβ(1–42) and huPrP(23–144) distributions in silver-stained Tris/Tricine SDS-PAGE gels and the distribution of RD2D3-FITC after fluorescence detection on the same gels are shown. B, D, and F, quantification by RP-HPLC of each component. Either huPrP(23–144) and RD2D3-FITC were simultaneously added to Aβ(1–42)oligo (A and B), huPrP(23–144) was preincubated with Aβ before RD2D3-FITC addition (C and D), or RD2D3-FITC was preincubated with Aβ before huPrP(23–144) addition (E and F). Dependent on the order of application of RD2D3-FITC or huPrP(23–144) to the sample, the distributions of huPrP(23–144) and RD2D3-FITC change. Experiments were done in replicates of n = 3 for all orders of application of RD2D3-FITC or huPrP(23–144) to the sample. Error bars represent S.D.