Figure 5.

β-Catenin protein, acetylation, and activity levels increase during osteogenesis. β-Catenin protein levels in C3H cells increase during osteogenesis, reaching significance at day 7 (D7) (A). After 7 days of osteoinduction, cells had increased levels of acetylated β-catenin as shown by immunoprecipitation (IP) (B) along with an increase in β-catenin activity as shown by luciferase assay (C). Blots are representative of two blots, and each blot contains three biological replicates. Data are means. Error bars represent S.E. (n = 3–6). *, p < 0.05 compared with day 0 (D0) control. IB, immunoblotting.