Figure 7.

(a) Bright field photograph of a squared microwell. (b) Regionalized hepatic C9 cells cultured onto PDMS replica, (yellow discontinued square corresponds to (c) and the red one corresponds to (d)). (c) In response to spatial confinement, cells upon/atop the microwell present YAP/TAZ (green) in the cytoplasm as shown by the non-colocalization with the nuclei stained with DAPI (blue). (d) On the contrary, cells outside the microwell, have YAP/TAZ in the nuclei, as shown by the colocalization with DAPI (full experiment unpublished at the time).