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The DTT effect on Nostoc sp. lysis for SC-WGA. After the heat-shock treatment, a mixture of 0.5 mM of EDTA, 200 U/µL of Ready-Lyse lysozyme, and the DTT of different concentrations was added to the cells and incubated at 37 °C for 2 h, followed by adding a D2 alkaline lysis buffer. N = 10 single cells per condition; error bars represent standard deviation.
