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. 2018 Jul 25;9(8):367. doi: 10.3390/mi9080367

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(a) Microscopic images of Nostoc sp., Sphaerocystis sp. and Gloeocapsa sp. The black boxes indicate additional cells from another capture to representatively illustrate cells in suspension. (b) The use of the developed lysis protocol on Sphaerocystis sp. and Gloeocapsa sp. for lysis and SC-WGA. N = 10 single cells per species per condition; error bars represent standard deviation. Each set of experiment was repeated three times. The sterile PBS as a true negative control did not show any contamination after amplification (not shown in the graph).