Fig. 1.

Spatiotemporal expression of GILZ during testicular development in wild type mice. Double immunolabelling for GILZ (green) and the germ cell marker GCNA (red) in the testis at various developmental stages: postnatal day (P) 0 (A–C), P5 (D–F), P10 (G–I) and adult P80 (J–O). A merged image of green and red is shown in the right panel. At P0 and P5, GILZ is expressed in the cytoplasm of developing spermatogonia. At P10, at the onset of meiosis, GILZ is present in the cytoplasm of B spermatogonia and a faint signal is detected in early prophase I spermatocytes localised in the centre of the seminiferous tubule. In adult testis, GILZ is expressed at the periphery of the tubule, showing expression in A spermatogonia (flattened basal pole), B spermatogonia, and in early spermatocytes (round cells). Scale bars = 20 µm.