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. 2018 Oct 10;11:6741–6756. doi: 10.2147/OTT.S167963

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© 2018 Pan et al. This work is published and licensed by Dove Medical Press Limited

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Overexpression of MTBP promotes lung adenocarcinoma cells invasion and migration both in vitro and in vivo.

Notes: (A and B) The expression level of MTBP mRNA and protein was measured following the treatment of HCC827 and PC9 cells with MTBP overexpression plasmids by qPCR and Western blot. (C) Representative images of Transwell migration assays (top panel) and Transwell invasion assays (bottom panel) in HCC827 and PC9 cells. Bar: 100 µm. (D) Average number of migrated or invasive cells per field of view from three independent experiments. (E) Representative images of cell migration analyzed by wound healing assay in HCC827 and PC9 cells. (F) Migratory distance of cells that migrated into the scratch wound at 6, 24, and 48 hours. (G and H) Representative image of tumors from CAM inoculated with HCC827 and PC9 cells and tumor weights. (I and J) Representative images of metastatic cells in the lungs of chicken embryos and the number of metastatic cells in the lungs of chicken embryos observed with a confocal laser scanning microscope. Data in (A, D and F) are the mean ± SD of results from three independent experiments. Data in (H and J) are the mean ± SD of results for each group. Student’s t-test, *P<0.05, **P<0.01, and ***P<0.001.

Abbreviations: CAM, chorioallantoic membrane; MTBP, MDM₂ binding protein; NC, normal control; qPCR, quantitative PCR.

Overexpression of MTBP promotes lung adenocarcinoma cells invasion and migration both in vitro and in vivo.

Notes: (A and B) The expression level of MTBP mRNA and protein was measured following the treatment of HCC827 and PC9 cells with MTBP overexpression plasmids by qPCR and Western blot. (C) Representative images of Transwell migration assays (top panel) and Transwell invasion assays (bottom panel) in HCC827 and PC9 cells. Bar: 100 µm. (D) Average number of migrated or invasive cells per field of view from three independent experiments. (E) Representative images of cell migration analyzed by wound healing assay in HCC827 and PC9 cells. (F) Migratory distance of cells that migrated into the scratch wound at 6, 24, and 48 hours. (G and H) Representative image of tumors from CAM inoculated with HCC827 and PC9 cells and tumor weights. (I and J) Representative images of metastatic cells in the lungs of chicken embryos and the number of metastatic cells in the lungs of chicken embryos observed with a confocal laser scanning microscope. Data in (A, D and F) are the mean ± SD of results from three independent experiments. Data in (H and J) are the mean ± SD of results for each group. Student’s t-test, *P<0.05, **P<0.01, and ***P<0.001.

Abbreviations: CAM, chorioallantoic membrane; MTBP, MDM₂ binding protein; NC, normal control; qPCR, quantitative PCR.