Skip to main content
. 2018 Oct 10;11:6777–6784. doi: 10.2147/OTT.S159337

Figure 3.

Figure 3

TRAF2 was targeted by miR-647.

Notes: (A) Two potential miR-647-binding sites in the TRAF2 3′ UTR were predicted using TargetScan 7.1; the mutant nucleotides are shown. A549 and H1299 cells were cotransfected with miR-647 mimics or the control vector with TRAF2-3′ UTR1, TRAF2-3′ UTR1-mut (B) or TRAF2-3′ UTR2, TRAF2-3′ UTR2-mut (C) for the Luciferase reporter assay. (D and E) A549 and H1299 cells were transfected with miR-647 mimics or the control vector to determine the mRNA and protein expression levels of TRAF2 by qRT-PCR and Western blotting. (F) The expression of TRAF2 was determined by qRT-PCR in 20 lung cancer tissue specimens that had undergone argon–helium cryoablation treatment or non-argon–helium cryoablation treatment. (G) Pearson’s correlation analysis indicated a negative correlation between the expression of miR-647 and TRAF2 (r = −0.744, P<0.01) *P<0.05.

Abbreviations: miR-647, microRNA-647; RT-PCR, quantitative real-time polymerase chain reaction; TRAF2, TNF receptor-associated factor 2; UTR, untranslated region.