Figure 1. Typical manipulation of Spheroid Catch.

(A) Schematic drawings for the Spheroid Catch protocol described in detail in the Results section. aSAS, bSAS, cSAS, dSAS, and eSAS denote the selected SAS cells after the first, second, third, fourth, and fifth selection rounds, respectively. (B) Typical microscopic images for the spheroids trapped by Spheroid Catch are highlighted by red circles (i) and an image of the selected spheroid incubated under tissue culture conditions (ii) is shown.