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. 2018 Sep 21;9(74):33947–33960. doi: 10.18632/oncotarget.26103

Figure 5. Tcf/LEF dependent β-catenin-signalling and target gene expression in hepatoma cells.

Figure 5

(a) Relative luciferase activity indicates the level of Tcf/LEF dependent β-catenin-signalling (grey bars) in parental (−), H1.3x and H1.3x− transfected Huh7 cells either treated with Dasatinib or DMSO. The ratio in parental cells was set to 1. Active viral replication was determined by measuring HBV rcDNA (white bars) levels in supernatants of HBV-producing cells. The assays were performed in independent triplicates, means + SD are shown. ** p<0.01 vs parental cells and HBV replicating cells respectively, t-test. (b) Immunofluorescence staining of β-catenin in parental (−) and HBV-producing H1.3 Huh7 cells either treated with Dasatinib or DMSO. Co-staining of HBV core protein and nuclei. Scale bar indicates 40μm. pgRNA and β-catenin-signalling dependent target gene mRNA were quantified by real-time PCR in (c) HBV infected HepG2-NTCP and (d) HBV infected Huh7.5-NTCP cells treated either with Dasatinib (grey bars) or Sorafenib (black bars) relative to DMSO treated HBV infected cells (white bars, ratios in DMSO treated cells were set to 1). Normalized expression ratios were relative to housekeeping gene GAPDH. The assays were performed in independent sixplicates, means + SD are shown. * p< 0.05; ** p<0.01 vs DMSO treated HBV infected cells, t-test.