Figure 2. CAI does not block IP3R calcium release but inhibits SOCE.

(A) Effect of CAI on IP3R and SOC. Microspectrofluorimetry using Fura-2AM probe was performed in the three carcinoma cell lines IGROV1-R10, OVCAR3 and SKOV3 cells. During exposure to 0Ca²⁺, depletion of the intracellular stores was triggered by the addition of 2 μM thapsigargin to the bathing medium. Subsequent replenishment of 2 mM Ca²⁺ to the medium elicited a rise in [Ca²⁺]_i due to Ca²⁺ influx through open SOC. Black tracings depict the representative changes in [Ca²⁺]_i recorded from DMSO-treated cells and grey tracings depict the representative changes in [Ca²⁺]_i recorded from cells pre-treated for 1h with 5μM CAI. (B) Means ± SEM of the peaks of thapsigargin-induced Ca²⁺ release and Ca²⁺ store-operated channel entry recorded from CAI pre-treated cells (black bar) or not (DMSO) (white bar) (n=3).