Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Oct 10;10:4421–4438. doi: 10.2147/CMAR.S176088

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Chen et al. This work is published and licensed by Dove Medical Press Limited

The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

PMC Copyright notice

LINE-1 ORF-1p enhanced the expression of PXR downstream genes in HCC cells.

Notes: (A and B) MHCC97-H cells were transfected with an empty vector or LINE-1 ORF-1p vectors. (C and D) MHCC97-H cells were transfected with control siRNA or LINE-1 ORF-1p siRNA. Cells were harvested for qPCR analysis. Relative mRNA levels for CYP3A4 (CYP/CYP450 group 3A type 4) (A and C) or MDR-1 (multidrug resistance) (D) were determined through qPCR. (E) MHCC97-H cells were transfected with an empty vector, LINE-1 ORF-1p, control siRNA or LINE-1 ORF-1p vectors. The protein level of LINE-1 ORF-1p, PXR, CYP3A4 or P-gp was identified through Western blotting. ^*P<0.05.

Abbreviations: HCC, hepatocellular carcinoma; PXR, pregnenolone X receptor; qPCR, quantitative PCR.